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At room temperature, media was removed, and cells were washed twice with 0.9% NaCl (aq)
The reduced PPIXpeptide (compound 1 ) and oxidized version with both Met residues oxidized to MetO (compound 2 ) were synthesized by using standard, microwave-assisted Fmoc solid-phase peptide synthesis, followed by an on-resin coupling of PPIX and purification through high-performance liquid chromatography (HPLC) after cleavage (Supplementary Figs
Meanwhile, DIO3 depletes the resources that can be utilized for the production of active thyroid molecules by catalyzing the conversion of T 4 and T 3 into inactive metabolites